Mediation of Epstein-Barr virus EBNA-LP transcriptional coactivation by Sp100.

نویسندگان

  • Paul D Ling
  • Rong Sheng Peng
  • Ayako Nakajima
  • Jiang H Yu
  • Jie Tan
  • Stephanie M Moses
  • Wei-Hong Yang
  • Bo Zhao
  • Elliott Kieff
  • Kenneth D Bloch
  • Donald B Bloch
چکیده

The Epstein-Barr virus (EBV) EBNA-LP protein is important for EBV-mediated B-cell immortalization and is a potent gene-specific coactivator of the viral transcriptional activator, EBNA2. The mechanism(s) by which EBNA-LP functions as a coactivator remains an important question in the biology of EBV-induced B-cell immortalization. In this study, we found that EBNA-LP interacts with the promyelocytic leukemia nuclear body (PML NB)-associated protein Sp100 and displaces Sp100 and heterochromatin protein 1alpha (HP1alpha) from PML NBs. Interaction between EBNA-LP and Sp100 was mediated through conserved region 3 in EBNA-LP and the PML NB targeting domain in Sp100. Overexpression of Sp100 lacking the N-terminal PML NB targeting domain, but not a mutant form of Sp100 lacking the HP1alpha interaction domain, was sufficient to coactivate EBNA2 in a gene-specific manner independent of EBNA-LP. These findings suggest that Sp100 is a major mediator of EBNA-LP coactivation. These studies indicate that modulation of PML NB-associated proteins may be important for establishment of latent viral infections, and also identify a convenient model system to investigate the functions of Sp100.

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عنوان ژورنال:
  • The EMBO journal

دوره 24 20  شماره 

صفحات  -

تاریخ انتشار 2005